We thank Dr. D. Chen and Dr. C. Yen for sharing the AFNI script to set up the real-time fMRI for PV 5 and the AFNI team for the software support. This research was supported by NIH Brain Initiative funding (RF1NS113278-01, R01 MH111438-01), and the S10 instrument grant (S10 RR023009-01) to Martinos Center, German Research Foundation (DFG) Yu215/3-1, BMBF 01GQ1702, and the internal funding from Max Planck Society.

This study was performed in accordance with the German Animal Welfare Act (TierSchG) and Animal Welfare Laboratory Animal Ordinance (TierSchVersV). The experimental protocol described here was reviewed by the ethics commission (&#167;15 TierSchG) and approved by the state authority (Regierungspr&#228;sidium, T&#252;bingen, Baden-W&#252;rttemberg, Germany).
1. Preparing the BOLD-fMRI experimental set-up for small animal study
<ol>
	<li>Turn on the console software to control imaging parameter...

<ol>
	<li>Ogawa, S., Lee, T. M., Kay, A. R., Tank, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain+magnetic+resonance+imaging+with+contrast+dependent+on+blood+oxygenation.">Brain magnetic resonance imaging with contrast dependent on blood oxygenation.</a> Proceedings of the National Academy of Sciences U.S.A. 87 (24), 9868-9872 (1990).</li><li>Belliveau, J. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+mapping+of+the+human+visual+cortex+by+magnetic+resonance+imaging.">Functional mapping of the human visual cortex by magnetic resonance imaging.</a> Science. 254 (5032), 716-719 (1991).</li><li>Stehling, M. K., Turner, R., Mansfield, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echo-planar+imaging:+magnetic+resonance+imaging+in+a+fraction+of+a+second.">Echo-planar imaging: magnetic resonance imaging in a fraction of a second.</a> Science. 254 (5028), 43-50 (1991).</li><li>Bandettini, P. A., Wong, E. C., Hinks, R. S., Tikofsky, R. S., Hyde, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Time+course+EPI+of+human+brain+function+during+task+activation.">Time course EPI of human brain function during task activation.</a> Magnetic Resonance in Medicine. 25 (2), 390-397 (1992).</li><li>Kwong, K. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+magnetic+resonance+imaging+of+human+brain+activity+during+primary+sensory+stimulation.">Dynamic magnetic resonance imaging of human brain activity during primary sensory stimulation.</a> Proceedings of the National Academy of Science U. S. A. 89 (12), 5675-5679 (1992).</li><li>Ogawa, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intrinsic+signal+changes+accompanying+sensory+stimulation:+functional+brain+mapping+with+magnetic+resonance+imaging.">Intrinsic signal changes accompanying sensory stimulation: functional brain mapping with magnetic resonance imaging.</a> Proceedings of the National Academy of Science U. S. A. 89 (13), 5951-5955 (1992).</li><li>Biswal, B., Yetkin, F. Z., Haughton, V. M., Hyde, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+connectivity+in+the+motor+cortex+of+resting+human+brain+using+echo-planar+MRI.">Functional connectivity in the motor cortex of resting human brain using echo-planar MRI.</a> Magnetic Resonance in Medicine. 34 (4), 537-541 (1995).</li><li>Logothetis, N. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What+we+can+do+and+what+we+cannot+do+with+fMRI.">What we can do and what we cannot do with fMRI.</a> Nature. 453 (7197), 869-878 (2008).</li><li>Kim, S. G., Ogawa, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biophysical+and+physiological+origins+of+blood+oxygenation+level-dependent+fMRI+signals.">Biophysical and physiological origins of blood oxygenation level-dependent fMRI signals.</a> Journal of Cerebral Blood Flow and Metabolism. 32 (7), 1188-1206 (2012).</li><li>Peltier, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+connectivity+changes+with+concentration+of+sevoflurane+anesthesia.">Functional connectivity changes with concentration of sevoflurane anesthesia.</a> Neuroreport. 16 (3), 285-288 (2005).</li><li>Dopfel, D., Zhang, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mapping+stress+networks+using+functional+magnetic+resonance+imaging+in+awake+animals.">Mapping stress networks using functional magnetic resonance imaging in awake animals.</a> Neurobiology of Stress. 9, 251-263 (2018).</li><li>Hu, X. P., Le, T. H., Parrish, T., Erhard, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retrospective+Estimation+and+Correction+of+Physiological+Fluctuation+in+Functional+Mri.">Retrospective Estimation and Correction of Physiological Fluctuation in Functional Mri.</a> Magnetic Resonance in Medicine. 34 (2), 201-212 (1995).</li><li>Birn, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+physiological+noise+in+resting-state+functional+connectivity.">The role of physiological noise in resting-state functional connectivity.</a> Neuroimage. 62 (2), 864-870 (2012).</li><li>Caballero-Gaudes, C., Reynolds, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methods+for+cleaning+the+BOLD+fMRI+signal.">Methods for cleaning the BOLD fMRI signal.</a> Neuroimage. 154, 128-149 (2017).</li><li>Pais-Roldan, P., Biswal, B., Scheffler, K., Yu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identifying+Respiration-Related+Aliasing+Artifacts+in+the+Rodent+Resting-State+fMRI.">Identifying Respiration-Related Aliasing Artifacts in the Rodent Resting-State fMRI.</a> Frontiers in Neuroscience. 12, 00788 (2018).</li><li>Glover, G. H., Li, T. Q., Ress, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Image-based+method+for+retrospective+correction+of+physiological+motion+effects+in+fMRI:+RETROICOR.">Image-based method for retrospective correction of physiological motion effects in fMRI: RETROICOR.</a> Magnetic Resonance in Medicine. 44 (1), 162-167 (2000).</li><li>Chang, C., Cunningham, J. P., Glover, G. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+heart+rate+on+the+BOLD+signal:+The+cardiac+response+function.">Influence of heart rate on the BOLD signal: The cardiac response function.</a> Neuroimage. 44 (3), 857-869 (2009).</li><li>Birn, R. M., Diamond, J. B., Smith, M. A., Bandettini, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Separating+respiratory-variation-related+neuronal-activity-related+fluctuations+in+fluctuations+from+fMRI.">Separating respiratory-variation-related neuronal-activity-related fluctuations in fluctuations from fMRI.</a> Neuroimage. 31 (4), 1536-1548 (2006).</li><li>Golestani, A. M., Chang, C., Kwinta, J. B., Khatamian, Y. B., Chen, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mapping+the+end-tidal+CO2+response+function+in+the+resting-state+BOLD+fMRI+signal:+Spatial+specificity,+test-retest+reliability+and+effect+of+fMRI+sampling+rate.">Mapping the end-tidal CO2 response function in the resting-state BOLD fMRI signal: Spatial specificity, test-retest reliability and effect of fMRI sampling rate.</a> Neuroimage. 104, 266-277 (2015).</li><li>Lu, H. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+animal+functional+magnetic+resonance+imaging+and+its+application+to+neurophamacological+studies.">Real-time animal functional magnetic resonance imaging and its application to neurophamacological studies.</a> Magnetic Resonance Imaging. 26 (9), 1266-1272 (2008).</li><li>Cox, R. W., Jesmanowicz, A., Hyde, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+functional+magnetic+resonance+imaging.">Real-time functional magnetic resonance imaging.</a> Magnetic Resonance Medicine. 33 (2), 230-236 (1995).</li><li>Lee, C. C., Jack, C. R., Rossman, P. J., Riederer, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+reconstruction+and+high-speed+processing+in+functional+MR+imaging.">Real-time reconstruction and high-speed processing in functional MR imaging.</a> American Journal of Neuroradiology. 19 (7), 1297-1300 (1998).</li><li>Voyvodic, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+fMRI+paradigm+control,+physiology,+and+behavior+combined+with+near+real-time+statistical+analysis.">Real-time fMRI paradigm control, physiology, and behavior combined with near real-time statistical analysis.</a> Neuroimage. 10 (2), 91-106 (1999).</li><li>Cohen, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+functional+magnetic+resonance+imaging.">Real-time functional magnetic resonance imaging.</a> Methods. 25 (2), 201-220 (2001).</li><li>Posse, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+approach+to+measure+single-event+related+brain+activity+using+real-time+fMRI:+Feasibility+of+sensory,+motor,+and+higher+cognitive+tasks.">A new approach to measure single-event related brain activity using real-time fMRI: Feasibility of sensory, motor, and higher cognitive tasks.</a> Human Brain Mapping. 12 (1), 25-41 (2001).</li><li>Decharms, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reading+and+controlling+human+brain+activation+using+real-time+functional+magnetic+resonance+imaging.">Reading and controlling human brain activation using real-time functional magnetic resonance imaging.</a> Trends in Cognitive Sciences. 11 (11), 473-481 (2007).</li><li>Bruhl, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Making+Sense+of+Real-Time+Functional+Magnetic+Resonance+Imaging+(rtfMRI)+and+rtfMRI+Neurofeedback.">Making Sense of Real-Time Functional Magnetic Resonance Imaging (rtfMRI) and rtfMRI Neurofeedback.</a> International Journal of Neuropsychopharmacology. 18 (6), (2015).</li><li>Cox, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=AFNI:+Software+for+analysis+and+visualization+of+functional+magnetic+resonance+neuroimages.">AFNI: Software for analysis and visualization of functional magnetic resonance neuroimages.</a> Computers and Biomedical Research. 29 (3), 162-173 (1996).</li><li>Liou, W. W., Goshgarian, H. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+assessment+of+the+effect+of+chronic+phrenicotomy+on+the+induction+of+the+crossed+phrenic+phenomenon.">Quantitative assessment of the effect of chronic phrenicotomy on the induction of the crossed phrenic phenomenon.</a> Experimental Neurology. 127 (1), 145-153 (1994).</li><li>Shih, Y. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultra+high-resolution+fMRI+and+electrophysiology+of+the+rat+primary+somatosensory+cortex.">Ultra high-resolution fMRI and electrophysiology of the rat primary somatosensory cortex.</a> Neuroimage. 73, 113-120 (2013).</li><li>Masamoto, K., Kim, T., Fukuda, M., Wang, P., Kim, S. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+neural,+vascular,+and+BOLD+signals+in+isoflurane-anesthetized+rat+somatosensory+cortex.">Relationship between neural, vascular, and BOLD signals in isoflurane-anesthetized rat somatosensory cortex.</a> Cerebral Cortex. 17 (4), 942-950 (2007).</li><li>van Alst, T. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anesthesia+differentially+modulates+neuronal+and+vascular+contributions+to+the+BOLD+signal.">Anesthesia differentially modulates neuronal and vascular contributions to the BOLD signal.</a> Neuroimage. 195, 89-103 (2019).</li><li>Wu, T. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+isoflurane+anesthesia+on+resting-state+fMRI+signals+and+functional+connectivity+within+primary+somatosensory+cortex+of+monkeys.">Effects of isoflurane anesthesia on resting-state fMRI signals and functional connectivity within primary somatosensory cortex of monkeys.</a> Brain and Behavior. 6 (12), 00591 (2016).</li><li>Liu, X., Zhu, X. H., Zhang, Y., Chen, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+change+of+functional+connectivity+specificity+in+rats+under+various+anesthesia+levels+and+its+neural+origin.">The change of functional connectivity specificity in rats under various anesthesia levels and its neural origin.</a> Brain Topography. 26 (3), 363-377 (2013).</li><li>Liu, X. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiphasic+modification+of+intrinsic+functional+connectivity+of+the+rat+brain+during+increasing+levels+of+propofol.">Multiphasic modification of intrinsic functional connectivity of the rat brain during increasing levels of propofol.</a> Neuroimage. 83, 581-592 (2013).</li><li>Hutchison, R. M., Hutchison, M., Manning, K. Y., Menon, R. S., Everling, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isoflurane+induces+dose-dependent+alterations+in+the+cortical+connectivity+profiles+and+dynamic+properties+of+the+brain's+functional+architecture.">Isoflurane induces dose-dependent alterations in the cortical connectivity profiles and dynamic properties of the brain's functional architecture.</a> Human Brain Mapping. 35 (12), 5754-5775 (2014).</li><li>He, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultra-Slow+Single-Vessel+BOLD+and+CBV-Based+fMRI+Spatiotemporal+Dynamics+and+Their+Correlation+with+Neuronal+Intracellular+Calcium+Signals.">Ultra-Slow Single-Vessel BOLD and CBV-Based fMRI Spatiotemporal Dynamics and Their Correlation with Neuronal Intracellular Calcium Signals.</a> Neuron. 97 (4), 925-939 (2018).</li><li>Yoshida, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physiological+effects+of+a+habituation+procedure+for+functional+MRI+in+awake+mice+using+a+cryogenic+radiofrequency+probe.">Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe.</a> Journal of Neuroscience Methods. 274, 38-48 (2016).</li></ol>

Sascha K&#246;hler is an employee at Bruker BioSpin MRI GmbH.

Real-time monitoring of the fMRI signal helps experimenters adjust the physiology of animals to optimize functional mapping. Motion artifacts in awake animals, as well as the anesthetic effect, are major factors that mediate the variability of fMRI signals, confounding the biological interpretation of the signal by itself31,32,33,34,35,<sup class="x...

Functional Magnetic Resonance Imaging (fMRI) is a non-invasive method to measure the hemodynamic responses1,2,3,4,5,6,7,8,9, e.g., the blood-oxygen-level-dependent (BOLD), cerebral blood volume and flow signal, associated with neural activity in the brain. In animal studies, hemodynamic signals can be affected by anesthesia10, the stress level of awake animals11, as well as the potential non-physiological artifacts, e.g., cardiac pulsation and respiratory motions12,13,14,15. Although many post-processing methods have been developed to provide a retrospective analysis of the fMRI signal for the task-related and resting-state functional dynamics and connectivity mapping16,17,18,19, there are few techniques to provide a real-time brain function mapping solution and instantaneous readouts in the animal brain20 (most of which are mainly used for human brain mapping21,22,23,24,25,26,27). In particular, this kind of real-time fMRI mapping method is lacking in animal studies. It is necessary to set up an fMRI platform to enable the investigation of real-time brain state-dependent physiological stages and to provides real-time biofeedback stimulation paradigm for animal brain functional studies.
In the present work, we illustrate a real-time fMRI experimental set-up with the customized macro-functions of the MRI console software, demonstrating real-time monitoring of the evoked BOLD-fMRI responses in the primary forepaw somatosensory cortex (FP-S1) of the anesthetized rats. This real-time set-up allows for the visualization of the ongoing brain activation in functional maps, as well as individual time courses in a voxel-wise manner, using the existing neuroimage analysis software, Analysis of Functional NeuroImages (AFNI)28. The preparation of the real-time fMRI experimental set-up for the animal study is described in the protocol. Besides the animal set-up, we provide detailed procedures to set up the visualization and analysis of the real-time fMRI signals using the latest console software in parallel with the image processing scripts. In summary, the proposed real-time fMRI set-up for animal studies is a powerful tool for monitoring the dynamic fMRI signals in the animal brain using the MRI console system.

<table>
	<tbody>
		<tr>
			<td>14.1T Bruker MRI system</td>
			<td>Bruker BioSpin MRI GmbH</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>A365 Stimulus Isolator</td>
			<td>World Precision Instruments</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>AcqKnowledge Software</td>
			<td>Biopac</td>
			<td>RRID:SCR_014279, http://www.biopac.com/product/acqknowledge-software/</td>
			<td></td>
		</tr>
		<tr>
			<td>AFNI</td>
			<td>Cox, 1996</td>
			<td>RRID:SCR_005927, http://afni.nimh.nih.gov</td>
			<td></td>
		</tr>
		<tr>
			<td>CO2SMO (ETCO2/SpO2 Monitor), Model 7100</td>
			<td>Novametrix Medical Systems Inc</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>CP-Pharma</td>
			<td>Cat# 1214</td>
			<td></td>
		</tr>
		<tr>
			<td>Master-9</td>
			<td>A.M.P.I</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Nanoliter Injector</td>
			<td>World Precision Instruments</td>
			<td>Cat# NANOFIL</td>
			<td></td>
		</tr>
		<tr>
			<td>Pancuronium Bromide</td>
			<td>Inresa Arzneimittel</td>
			<td>Cat# 34409.00.00</td>
			<td></td>
		</tr>
		<tr>
			<td>ParaVision 6</td>
			<td>Bruker BioSpin MRI GmbH</td>
			<td>RRID:SCR_001964</td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphate Buffered Saline (PBS)</td>
			<td>Gibco</td>
			<td>Cat# 10010-023</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat: Sprague Dawley rat</td>
			<td>Charles River Laboratories</td>
			<td>Crl:CD(SD)</td>
			<td></td>
		</tr>
		<tr>
			<td>SAR-830/AP Ventilator</td>
			<td>CWE</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>&#945;-chloralose</td>
			<td>Sigma-Aldrich</td>
			<td>Cat# C0128-25G;RRID</td>
			<td></td>
		</tr>
	</tbody>
</table>

real-time fmri brain mapping in animals

Dynamic fMRI responses vary largely according to the physiological conditions of animals either under anesthesia or in awake states. We developed a real-time fMRI platform to guide experimenters to monitor fMRI responses instantaneously during acquisition, which can be used to modify the physiology of animals to achieve desired hemodynamic responses in animal brains. The real-time fMRI set-up is based on a 14.1T preclinical MRI system, enabling the real-time mapping of dynamic fMRI responses in the primary forepaw somatosensory cortex (FP-S1) of anesthetized rats. Instead of a retrospective analysis to investigate confounding sources leading to the variability of fMRI signals, the real-time fMRI platform provides a more effective scheme to identify dynamic fMRI responses using customized macro-functions and a common neuroimage analysis software in the MRI system. Also, it provides immediate troubleshooting feasibility and a real-time biofeedback stimulation paradigm for brain functional studies in animals.

Figure 3 and Figure 4 show a representative real-time voxel-wise BOLD-fMRI time course and functional maps with electrical forepaw stimulation (3 Hz, 4 s, pulse width 300 us, 2.5 mA). The fMRI design paradigm comprises 10 pre-stimulation scans, 3 stimulation scans, and 12 inter-stimulation scans with a total of 8 epochs (130 scans). The total scan time is 3 min 15 sec (195 sec). Figure 3 shows the voxel-wise time course (black line)...

Watch this Scientific Journal Video about Real-Time fMRI Brain Mapping in Animals at JoVE.com

Real-Time fMRI Brain Mapping in Animals

Animal brain functional mapping can benefit from the real-time functional magnetic resonance imaging (fMRI) experimental set-up. Using the latest software implemented in the animal MRI system, we established a real-time monitoring platform for small animal fMRI.

Dynamic fMRI responses vary largely according to the physiological conditions of animals either under anesthesia or in awake states. We developed a ...

Instead of retrospective analysis to investigate compounding sources. The proposed leader time fMRI platform provides a more effective scheme for identifying dynamic fMRI response using the latest console software. This technique can provide immediate troubleshooting feasibility and a real-time biofeedback simulation paradigm for brain functional studies in animals To acquire anatomical images covering the whole brain in the coronal view, click the continue button to run a T2 weighted rare sequence.To set up the realtime fMRI software. Open a terminal window and use the command to navigate to the realtime AFNI plugin path. Use the commands as indicated to execute the AFNI software with the realtime plugin.Use the diamond command and options to monitor the upcoming AFNI brick files. Use the PVC MD command with the options as indicated, to run the background macro script. To obtain the EPI acquisition parameters, use the exec PVC MD command and options.To convert the 3D ECHO planar imaging raw data, to AFNI files, in real time, in the background macro script. Use the exec TOD-3D Command and options, making sure that EPI geometrical information is consistent with the anatomy orientation. Click the continue button to run a T2 Star Weighted 3D EPI Sequence for bold fMRI imaging.To overlay the EPI images, onto the anatomical rare images. First, use the command to convert the rare images to an AFNI Brick dataset and use the script and options to register the EPI images to the anatomical images. To process resting-state functional connectivity maps of the bold responses.Use AFNI options to calculate the seed based correlation of the 3D plus time dataset, such as resting state fMRI time series. Then to visualize functional maps of the bold signals, select the defined overlay option and use the clusters function, from the AFNI user interface menu, to generate an interactive clustering in the AFNI software. To visualize response electrical stimulation responses by fMRI.Turn on an electrical stimulus isolator and use stimulation blocks to perform electrical four-paw stimulation for one evoked fMRI analysis. In this Representative Real-Time Voxel-Wise Bold fMRI Time Course. The voxel-wise time course of the contralaterally primary four-paw somatosensory cortex, corresponding to the block design paradigm in the real-time acquisition format, after electrical four-paw stimulation can be observed.As illustrated activated bold maps corresponding to the electrical four-paw stimulation can also be generated with the activated regions detected and displayed as colored clusters. Be sure to activate the 3D EPI Reconstruction option pre-image 3D activity and execute macro in the data construction user interface menu. And to link to the predefining macro script set-up RT 3D API before scanning.

Research

JoVE Journal

Neuroscience

3.3K Görüntüleme.  Max Planck Institute for Biological Cybernetics. Instead of retrospective analysis to investigate compounding sources. The proposed leader time fMRI platform provides a more effective scheme for identifying dynamic fMRI response using the latest console software. This technique can provide immediate troubleshooting feasibility and a real-time biofeedback simulation paradigm for brain functional studies in animals To acquire anatomical images covering the whole brain in the coronal view, click the continue button to run a T2 weighted rare se...