Murine Hepatocyte Isolation by Liver Perfusion and Digestion
2:47
Hepatocyte Organoids Culture
4:29
Single Cells from Hepatocyte Organoids
7:22
siRNA or Plasmid Transfection
12:58
Representative Results
13:58
Conclusion
Transkript
The liver is the biggest organ in mammals. It plays very important role in metabolism and detoxification. Although the liver has a remarkable regeneration capacities in vitro, it is still a very big challenge for a long term to culture hepatocyte
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A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering.