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Overview

1:40

Principles of Nitrate and Phosphate Detection

3:40

Measuring Nitrogen in a Sample

5:11

Measuring Phosphorus in a Sample

7:11

Results

8:29

Applications

10:25

Summary

Nutrients in Aquatic Ecosystems

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University

Nitrogen and phosphorus are essential plant nutrients found in aquatic ecosystems and both are monitored as a part of water quality testing because in excess amounts they can cause significant water quality problems. 

Nitrogen in water is measured as the common form nitrate (NO3-) that is dissolved in water and readily absorbed by photosynthesizers such as algae. The common form of phosphorus measured is phosphate (PO43-), which is strongly attracted to sediment particles as well as dissolved in water. In excess amounts, both nutrients can cause an increase in aquatic plant growth (algal bloom, Figure 1) that can disrupt the light, temperature, and oxygen levels in the water below and lead to eutrophication and hypoxia (low dissolved oxygen in water) forming a “dead zone” of no biological activity. Sources of nitrates and phosphorus include wastewater treatment plants, runoff from fertilized lawns and agricultural lands, faulty septic systems, animal manure runoff, and industrial waste discharge.

Figure 1
Figure 1. Algal bloom
Taken in 2011, the green scum shown in this image was the worst algae bloom Lake Erie has experienced in decades. Record torrential spring rains washed fertilizer into the lake, promoting the growth of microcystin producing cyanobacteria blooms. Vibrant green filaments extend out from the northern shore.

1. Measure Nitrogen in Sample

  1. On the spectrophotometer, find the program for nitrate (with user manual or instrument menu) and enter the program number.  
  2. Pipette 10 mL of the water sample into one of the sample tubes. Pour this into one of the sample tubes.
  3. Repeat for a second sample tube.
  4. Add the contents of one nitrate reagent powder pillow to one sample tube.
  5. Cap both sample tubes.
  6. On the spectrophotometer, press timer and enter to start a r

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Figure 2
Figure 2. Graph comparing nitrates between different land use types (undeveloped, agricultural, and urban).

Average nitrate concentrations compared upstream and downstream from a water treatment plant (Figure 3). The downstream measurement represents the discharge from the treatment.

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High concentrations of nitrates and phosphorus can stimulate eutrophic conditions in water by causing algal bloom that negatively affects other water quality factors including dissolved oxygen, temperature, and other indicators. Excess nitrates can lead to hypoxic water (low levels of dissolved oxygen) no longer able to support aerobic life creating a “dead zone,” where non-mobile species mass die-offs and mobile species move away to other waters. Dead zones are occurring globally in coastal regions where lar

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