Sign In

Water Quality Analysis via Indicator Organisms

Overview

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba -The University of Arizona
Demonstrating Author: Luisa Ikner

Water quality analysis monitors anthropogenic influences such as pollutants, nutrients, pathogens, and any other constituent that can impact the water’s integrity as a resource. Fecal contamination contributes microbial pathogens that threaten plant, animal, and human health with disease or illness. Increasing water demands and strict quality standards require that water being supplied for human or environmental resources be monitored for low pathogen levels. However, monitoring each pathogen associated with fecal pollution is not feasible, as laboratory techniques involve extensive labor, time, and costs. Therefore, detection for indicator organisms provides a simple, rapid, and cost effective technique to monitor pathogens associated with unsanitary conditions.

Procedure

1. Colilert Presence – Absence (P – A) Test

  1. Open the 100 mL plastic Colilert bottle. The bottle includes a small amount of powdered reagent that is necessary for the proper reactions, do not discard this powder.
  2. Add 100 mL water sample into Colilert bottle.
  3. Open the pillow tube containing the nutrient substrate and pour the contents into the water sample inside the Colilert bottle.
  4. Cap and seal the Colilert bottle. Shake the bottle vigorously, repeatedly inverting the

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Application and Summary

Indicator organisms are employed to rapidly and inexpensively determine environmental contamination. Colilert assays are utilized to analyze water quality for drinking, recreational, and wastewater sources. Water quality must meet legal standards set by the Environmental Protection Agency (EPA) and state regulatory departments in order to be accepted as a resource for human and/or environmental consumption.

Colilert assays are also strategically used as mass balance markers within environmenta

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Tags
Water Quality AnalysisIndicator OrganismsFecal MatterDisease causing PathogensWater SuppliesFecal ContaminationGastrointestinal PathogensMonitoring Water SamplesUnique PathogensFecal PollutionWater ResourcesWater Quality IndicatorDetectable OrganismsPathogen LevelsEnvironmental PersistenceEasy DetectionSafe DetectionInexpensive DetectionTotal ColiformsFecal Coliforms

Skip to...

0:00

Overview

1:10

Principles of Monitoring Water Quality Using Indicator Organisms

3:49

Coliform Presence-Absence (P-A) Test

5:06

Most Probable Number (MPN) Analysis

6:44

Applications

7:54

Summary

Videos from this collection:

article

Now Playing

Water Quality Analysis via Indicator Organisms

Environmental Microbiology

28.9K Views

article

Determination of Moisture Content in Soil

Environmental Microbiology

355.3K Views

article

Aseptic Technique in Environmental Science

Environmental Microbiology

121.6K Views

article

Gram Staining of Bacteria from Environmental Sources

Environmental Microbiology

96.0K Views

article

Visualizing Soil Microorganisms via the Contact Slide Assay and Microscopy

Environmental Microbiology

41.2K Views

article

Filamentous Fungi

Environmental Microbiology

54.1K Views

article

Community DNA Extraction from Bacterial Colonies

Environmental Microbiology

28.5K Views

article

Detecting Environmental Microorganisms with the Polymerase Chain Reaction and Gel Electrophoresis

Environmental Microbiology

43.6K Views

article

RNA Analysis of Environmental Samples Using RT-PCR

Environmental Microbiology

40.0K Views

article

Quantifying Environmental Microorganisms and Viruses Using qPCR

Environmental Microbiology

47.4K Views

article

Isolation of Fecal Bacteria from Water Samples by Filtration

Environmental Microbiology

37.2K Views

article

Detection of Bacteriophages in Environmental Samples

Environmental Microbiology

40.2K Views

article

Culturing and Enumerating Bacteria from Soil Samples

Environmental Microbiology

180.5K Views

article

Bacterial Growth Curve Analysis and its Environmental Applications

Environmental Microbiology

293.0K Views

article

Algae Enumeration via Culturable Methodology

Environmental Microbiology

13.6K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved