Name: drosophila larval nmj immunohistochemistry
Uploaded: 2009-03-28T00:00:00
Description: Watch this Scientific Journal Video about Drosophila Larval NMJ Immunohistochemistry at JoVE.com

Prima di iniziare <ol><li> Preparare le seguenti soluzioni: PBT (0,1% Triton X100 in PBS 1X), PBTB (0,2% BSA in PBT), e PBTN (2% in PBTB NGS). </li><li> Sezionare larve di Drosophila. Si prega di consultare Drosophila larvale Dissection NMJ. </li></ol> Immunoistochimica <ol><li> Spostare le larve in una provetta contenente 1,5 ml PBT. Lavare le larve due volte per 15 minuti in PBT. Nota: per lavare, posizionare il tubo di 1,5 ml di un mixer nutator. Asportare il liqu...

<ol>
	<li>Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Properties+of+the+larval+neuromuscular+junction+in+Drosophila+Melanogaster.">Properties of the larval neuromuscular junction in Drosophila Melanogaster.</a> J. Physiol. 262, 189-214 (1976).</li><li>Johansen, J., Halpern, M. E., Johansen, K. M., Keshishian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stereotypic+morphology+of+glutamatergic+synapses+on+identified+muscle+cells+of+Drosophila+larvae.">Stereotypic morphology of glutamatergic synapses on identified muscle cells of Drosophila larvae.</a> J Neurosci. 9, 710-725 (1989).</li><li>Kesheshian, H., Broadie, K., Chiba, A., Bate, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+neuromuscular+junction:+a+model+system+for+studying+synaptic+development+and+function.">The Drosophila neuromuscular junction: a model system for studying synaptic development and function.</a> Annu Rev Neurosci. 19, 545-575 (1996).</li><li>Collins, C. A., DiAntonio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+development:+insights+from+Drosophila.">Synaptic development: insights from Drosophila.</a> Curr Opin Neurobiol. 17 (1), 35-42 (2007).</li></ol>

Immunoistochimica (IHC) è di vitale importanza per lo studio della biologia NMJ perché consente la visualizzazione delle NMJ. Questo è ottenuto utilizzando anticorpi che riconoscono la membrana neuronale (ad esempio, HRP), il presynapse (ad esempio, CSP, SYT), e / o il postsynapse (ad esempio, DLG). Molecole di segnalazione, proteine ​​strutturali, nuove proteine ​​o di interesse possono anche essere colorati. Poi, i geni possono essere mutati o missexpressed e IHC in grado di rilevare perturbazione di strutt...

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drosophila larval nmj immunohistochemistry

Il<em&gt; Drosophila</em&gt; Giunzione neuromuscolare (NMJ) è stabilito un sistema modello utilizzato per lo studio dello sviluppo e della plasticità sinaptica. L&#39;uso diffuso del<em&gt; Drosophila</em&gt; Motore del sistema è dovuta alla sua alta accessibilità. Può essere analizzati con cella singola risoluzione. Ci sono 30 muscoli per hemisegment la cui disposizione all&#39;interno delle mura organo periferico sono noti. Un totale di 31 neuroni motori attribuiscono a questi muscoli in un modello che ha alta fedeltà. Utilizzando biologia molecolare e della genetica, si possono creare animali transgenici o mutanti. Poi, si può studiare le conseguenze dello sviluppo sulla morfologia e la funzione del NMJ. Immunoistochimica può essere usato per l&#39;immagine chiaramente i componenti del NMJ. In questo articolo mostriamo come utilizzare colorazione anticorpi per visualizzare le<em&gt; Drosophila</em&gt; Larvale NMJ.

Watch this Scientific Journal Video about Drosophila Larval NMJ Immunohistochemistry at JoVE.com

Drosophila Larval NMJ Immunohistochemistry

Questo protocollo illustra come eseguire immunoistochimica su sezionato larva di Drosophila.

Drosophila larvali NMJ Immunoistochimica

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

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Biology

Drosophila Larval NMJ Immunohistochemistry

Dissection of Larval CNS in Drosophila Melanogaster

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The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

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Sezionamento sottile fetta di preparativi per immunoistochimica

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In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. ...

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We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

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Many organs depend on stem cells for their development during embryogenesis and for maintenance or repair during adult life. Understanding how stem cells ...

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The Drosophila pupal abdomen is an established model system for the study of epithelial morphogenesis and the development of sexually dimorphic ...

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Cell shape is critical for cell function. However, despite the importance of cell morphology, little is known about how individual cells generate specific ...

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Studies performed in Drosophila melanogaster embryos and larvae provide crucial insight into developmental processes such as cell fate specification and ...