Name: mating and tetrad separation of <em>chlamydomonas reinhardtii</em> for genetic analysis
Uploaded: 2009-08-12T19:00:00
Description: Watch this Scientific Journal Video about Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis at JoVE.com

<p class="jove_content">该协议是基于在实验室研究所生物学物理化学公司，巴黎，法国的弗朗西斯，安德烈沃尔曼学到的技术。作者欠报恩韦芝Bascou，原本星展，以基因工程技术生产的<em>衣，</em>衣在作者实验室的研究是由美国国家科学基金会奖MCB - 0646350的支持。 </p>

<p class="jove_title">第1部分：配子株的制备</p><p class="jove_content">注：所有的操作应在室温下进行。更高或更低的温度会影响受精卵萌发的速度和可能产生不利影响的可行性。 <br />重要的考虑因素：重要的是要交配的菌株是增长强劲，真菌或细菌污染。如果菌株没有经常被转移，通过丰富培养基上应先产生配子。 </p><ol><li>要交配的菌株转移到抽头板（100 × 15毫米），每盘1-2株。细胞生长在一个约1厘米宽的集中地带。 ...

<ol>
	<li>Harris, E. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Chlamydomonas+Sourcebook:+Introduction+to+Chlamydomonas+and+Its+Laboratory+Use.">The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use.</a> <em style='font-style: italic'>Conf Proc IEEE Eng Med Biol Soc</em>. , 119-273 (2005).</li></ol>

<p class="jove_content">遗传分析是简单的在<em>衣</em> ，但它需要特别的技术，以产生配子和独立的四分体后代。与健康株，这些技术很容易掌握。一些突变株，它成为一门艺术，和读者应参考最近出版的<em>衣</em>历史笔记<em>的原始</em>资料和实用提示第1卷。此外，应该指出的是，有许多变化的基本主题，如上所述，从方法诱导配子，受精卵分布和解剖模式。最终，每个研究员认为自己的舒适区，或适应实验室专用协?...

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<td>CC-125</td>
<td>Mating type <em>plus</em> strain</td>
<td><em>Chlamydomonas</em> Stock Center</td>
<td>&nbsp;</td>
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<td><em>Chlamydomonas</em> Stock Center</td>
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mating and tetrad separation of <em>chlamydomonas reinhardtii</em> for genetic analysis

<p class="jove_content">单细胞绿藻<em&gt;衣藻</em&gt;（<em&gt;衣</em&gt;）已成为一个流行的有机体，在不同的领域，由于其简单的生命周期，细胞生物学和遗传学的研究，缓解遗传分析，基因资源，原子核transformability和两个细胞器的增长和操纵。当遗传的方法是使用在交配菌株是一种常见的做法<em&gt; Chlamydomonass</em创建主导地位的营养分析二倍体，或四分体夹层，以确定核与细胞器的继承，测试等位，分析epistasy，或生成的图位克隆的目的的人群。此外，遗传杂交经常使用结合起来，特别是核基因型的细胞器基因型。在这里，我们展示了标准方法对配子，交配，合子萌发和四分体分离。该协议包括一个易于遵循的一系列步骤，这将使科学家所不熟悉的遗传方法适合<em&gt;衣</em&gt;。关键参数和故障点的解释。最后，提供进一步的信息和替代方法的资源。</p>

Watch this Scientific Journal Video about Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis at JoVE.com

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

<p class="jove_content">交配和四分体分离的遗传分析<em&gt;衣藻</em&gt;。在这里，我们展示了标准方法对配子，交配，合子萌发和四分体夹层。该协议包括一个易于遵循的一系列步骤，这将使科学家所不熟悉的遗传方法适合<em&gt;衣</em&gt;。</p>

交配和四分体分离<em>衣藻</em>遗传分析

The unicellular green alga Chlamydomonas reinhardtii (Chlamydomonas) has become a popular organism for research in diverse areas of cell biology and ...

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Mating and Tetrad Separation of <em>Chlamydomonas reinhardtii</em> for Genetic Analysis

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Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Agarose is isolated from ...

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Human pluripotent stem cells (hPSCs) hold great promise for regenerative medicine and biopharmaceutical applications. Currently, optimal culture and ...

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Mature muscle has a unique structure that is amenable to live cell imaging. Herein, we describe the experimental protocol for expressing fluorescently ...

交配和四分体分离衣藻遗传分析