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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

10.0K Views

15:41 min

October 12th, 2009

October 12th, 2009

9,977 Views

0:00

Title

0:44

Introduction

1:02

Growing Isotopically Light and Isotopically Heavy Yeast Cells

3:06

Lysing Cells

6:35

Isolation and Fractionation of Peptides

6:38

Alkylation and Reduction

10:32

Desalting

11:54

Peptide Fractionation

13:11

Enrichment of Phosphopeptides

14:51

Representative Results

15:14

Conclusion

Description of a quantitative phosphorylation procedure using cryolysis, urea solubilziation, HILIC fractionation and IMAC enrichment of phosphorylated peptides.

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