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Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

10.2K Views

13:52 min

July 9th, 2013

DOI :

10.3791/50432-v

July 9th, 2013


Transcript

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Keywords Protein Refolding

Chapters in this video

0:05

Title

1:46

Construction of Strains Containing FFL-GFP Plasmid

2:23

Preparations Prior to FFL-GFP Induction and Enzymatic Recovery Assay

3:48

Induction of FFL-GFP

6:22

Enzymatic FFL-GFP Recovery Assay

7:35

Fluorescence Microscopy

8:55

Single Cell Microscopy

10:47

Results: Hsp104 is Required for Efficient Refolding of Heat-denatured FFL-GFP

13:36

Conclusion

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