JoVE Logo
Faculty Resource Center

Sign In

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

DOI :

10.3791/50432-v

July 9th, 2013

July 9th, 2013

10,125 Views

1Department of Microbiology and Molecular Genetics, Graduate School of Biomedical Sciences, University of Texas Medical School

This article describes the use of a firefly luciferase-GFP fusion protein to investigate in vivo protein folding in Saccharomyces cerevisiae. Using this reagent, refolding of a model heat-denatured protein can be monitored simultaneously by fluorescence microscopy and an enzymatic assay to probe the roles of proteostasis network components in protein quality control.

-- Views

Related Videos

article

Dissection of Saccharomyces Cerevisiae Asci

article

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

article

Microarray Analysis for Saccharomyces cerevisiae

article

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

article

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

article

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

article

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

article

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

article

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

article

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved