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Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

DOI :

10.3791/50432-v

July 9th, 2013

July 9th, 2013

10,095 Views

1Department of Microbiology and Molecular Genetics, Graduate School of Biomedical Sciences, University of Texas Medical School

This article describes the use of a firefly luciferase-GFP fusion protein to investigate in vivo protein folding in Saccharomyces cerevisiae. Using this reagent, refolding of a model heat-denatured protein can be monitored simultaneously by fluorescence microscopy and an enzymatic assay to probe the roles of proteostasis network components in protein quality control.

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