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Identification of protein complexes with quantitative proteomics in S. cerevisiae

DOI :

10.3791/1225-v

March 4th, 2009

March 4th, 2009

13,059 Views

1Department of Cellular and Physiological Sciences, University of British Columbia - UBC, 2Department of Biochemistry and Molecular Biology, University of British Columbia - UBC

Here we describe a new quantitative proteomics technique for identifying protein complexes in Saccharomyces cerevisiae. In this study, we have used the SILAC method together with affinity purification followed by tandem mass spectrometry to identify with high specificity the binding partners of an ER protein, Scs2p.

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Protein Complexes

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