JoVE Logo
Faculty Resource Center

Sign In

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

DOI :

10.3791/1542-v

10:57 min

November 2nd, 2009

November 2nd, 2009

12,567 Views

1Physics Department, Boston University, 2Department of Biomedical Engineering, Boston University

In this article we describe how we obtain FRET traces from individual DNA molecules immobilized to a surface using an automated scanning confocal microscope.

Tags

Automated System

-- Views

Related Videos

article

Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

article

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

article

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

article

Single-Molecule Imaging of Nuclear Transport

article

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

article

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

article

Studying DNA Looping by Single-Molecule FRET

article

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

article

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

article

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved