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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

DOI :

10.3791/1529-v

15:57 min

October 9th, 2009

October 9th, 2009

21,996 Views

1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

Tags

Single molecule DNA Replication

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