Results: NdeI Cleavage is Dependent on Concentrations of Protein and Magnesium
6:14
Conclusion
Transcript
This protocol can be applied to any process that results in double stranded DNA breaks, yields detailed quantitative data on kinetics. Although this method has single molecule resolution, it measures up to 1000 DNAs in a single experiment, thus pr
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A highly parallel method for measuring the site-specific cleavage of DNA at the single molecule level is described. This protocol demonstrates the technique using the restriction endonuclease NdeI. The method can easily be modified to study any process that results in site-specific DNA cleavage.