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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

DOI :

10.3791/1662-v

12:55 min

January 7th, 2010

January 7th, 2010

23,542 Views

1Gene Expression Division, Bio-Rad Laboratories, Inc.

This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.

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Gene Pulser MXcell Electroporation System

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