Cell Preparation, Experimental Setup, Operation, and Analysis
8:24
Results: Cell Membrane Permeabilization and Electro‐Transfection Efficiency Using the Continuous Flow, Micro‐Electroporation System
9:36
Conclusion
Transcript
This method allows researchers to determine optimal electroporation pulsing conditions for the desired cell type, while minimizing the need for the traditional empirical experimental approach. This method utilizes micro-electroporation techniques
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This protocol describes the microfabrication techniques required to build a lab-on-a-chip, microfluidic electroporation device. The experimental setup performs controlled, single-cell-level transfections in a continuous flow and can be extended to higher throughputs with population-based control. An analysis is provided showcasing the ability to electrically monitor the degree of cell membrane permeabilization in real-time.