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Development of automated imaging and analysis for zebrafish chemical screens.

11.3K Views

10:49 min

June 24th, 2010

June 24th, 2010

11,264 Views

0:00

Title

0:53

Introduction

1:55

Set Up Zebrafish Matings and BCl Treatment

4:47

Automated Imaging of 96-Well Plates and Analysis

9:06

Representative Results

9:46

Conclusion

We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

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