JoVE Logo
Faculty Resource Center

Sign In

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

DOI :

10.3791/2686-v

June 6th, 2011

June 6th, 2011

13,474 Views

1Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 2Macromolecular Therapeutics Development Facility, Albert Einstein College of Medicine, Yeshiva University, 3Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University

A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.

Tags

Glycans

-- Views

Related Videos

article

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

article

Generating Chimeric Zebrafish Embryos by Transplantation

article

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

article

Imaging Cell Shape Change in Living Drosophila Embryos

article

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

article

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

article

Metabolic Profile Analysis of Zebrafish Embryos

article

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

article

Analysis of Oxidative Stress in Zebrafish Embryos

article

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved