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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

DOI :

10.3791/3448-v

10:58 min

February 27th, 2012

February 27th, 2012

17,228 Views

1Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée

We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.

Tags

Quantitative

-- Views

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