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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

DOI :

10.3791/1960-v

8:11 min

May 19th, 2010

May 19th, 2010

14,950 Views

1Department of Cell Biology and Human Anatomy Institute for Pediatric Regenerative Medicine, School of Medicine, University of California, Davis

We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.

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Embryonic Stem Cells

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