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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

DOI :

10.3791/56358-v

December 3rd, 2017

December 3rd, 2017

10,037 Views

1Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, 2Department of Psychiatry, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine

This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

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Mouse Embryonic Stem Cells

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