December 3rd, 2017
•This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.
Related Videos
Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
Isolation and Culture of Mouse Cortical Astrocytes
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved