To begin, change into long-sleeved scrub top or short-sleeved scrub top with non-sterile sleeves, full-length scrub pants, and cleanroom shoes with shoe covers on. Sanitize hands with an alcohol-based hand sanitizer, and don non-sterile gloves, a beard cover if applicable, and a non-sterile bouffant. Remove the shoe covers, and step onto the tacky mat in front of the International Organization for Standardization or ISO-8 anteroom door.
After discarding the shoe covers, decontaminate the gloves and door handle with 70%sterile isopropyl alcohol before entering the ISO 8 anteroom. Gather a sterile hood, face mask, coveralls, boot covers, and safety glasses. After decontaminating the gloves again, don safety glasses and stage the materials onto a clean surface.
Don a new pair of non-sterile shoe covers over the dedicated cleanroom shoes, step onto the tacky mat, and enter the secondary ISO 7 anteroom, remaining on the dirty side of the demarcation line. Don the sterile hood and the sterile coverall, ensuring the neck of the hood is tucked inside the coverall to create a complete seal. Don the secondary sterile boot covers and decontaminate the gloves between each donning step before entering the ISO 7 space.
To don into the biological safety cabinet, don sterile sleeves over the coverall, using thumb loops if present. Don sterile gloves over the existing gloves, ensuring the cuff of the glove extends over the sterile sleeve before entering the biological safety cabinet.
