Installing the Live Imaging Insert on an Inverted Microscope Stage

To begin installing the 3D printed live imaging insert on the inverted microscope stage, carefully place the insert into the large microscope stage groove. Using screws, secure each corner of the insert. Slide the heat plate into the side opening of the insert in a plug side down orientation, so the plate lays atop the lower insert grooves with the plug port underneath the stage.

Align the grooved circular opening in the insert with a 40 x silicone oil immersion objective and apply silicone oil to the center of the objective. Then using a syringe, apply a small amount of vacuum grease along the grooved circular opening. Lay the cover slip disk on top of the opening to seal it onto the insert.

Finally, raise the 40 x objective until it barely touches the bottom of the glass cover slip.