Methods Article

Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

1.5K Views

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04:11 min

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June 30th, 2023

DOI :

10.3791/65529-v

June 30th, 2023

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Michael Hamersky IV¹, Khushi Tekale¹, L. Matthew Winfree², Matthew J. M. Rowan¹^,³, Lindsey Seldin¹^,⁴^,⁵^,⁶

¹Department of Cell Biology, Emory University School of Medicine, ²Independent scholar, ³Center for Neurodegenerative Disease, Emory University School of Medicine, ⁴Department of Dermatology, Emory University School of Medicine, ⁵Winship Cancer Institute, Emory University School of Medicine, ⁶Atlanta Veterans Affairs Medical Center

Transcript

Summary

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Intravital Imaging

Inverted Confocal Microscope

Skin Cell Dynamics

Multiphoton Microscopy

Imaging Versatility

Cell Tracking

Disease Progression

3D printed Microscope Stage

Epithelial Tissue

Dermal Cell Behaviors

Noninvasive Microscopy

Transgenic Mice

Clinical Interventions

Skin Carcinomas

Chapters in this video

0:00

Introduction

1:08

Installing the Live Imaging Insert on an Inverted Microscope Stage

2:05

Mounting the Mouse on the Live Imaging Insert for Intravital Imaging

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