After developing the frozen shoulder rat model, manipulate the mechanoreceptor and force parameters in three directions using the software. Next, use the thumb to perform the rotatory kneading method with a rotating motion. Add 0.5 kilograms of strength and a frequency of 100 to 120 times per minute.
Use the thumb fingertip to perform the point pressing method at a strength of 0.5 kilograms. To perform tuina, hold the rat until it calms down and perform the manipulation. Place the rat in the lateral recumbent position, adjusting as needed for the specific manipulation method.
Using the right index and middle fingers, clamp the right forelimb of the rat. Flex and extend it several times to determine the positions of the rat's shoulder joint, elbow joint, and humerus. Then place the subject in a lateral recumbent position and manipulate the forelimb muscles.
Manipulate the shoulder and back muscles in the prone position. Now use the rat acupoint atlas to determine the location of each acupoint. Press the LI 15 acupoint found in the depression anterior-inferior to the acromial end while in the prone position.
Next, press the SI 11 acupoint in the depression at the infraspinatus fossa, the midpoint of the scapular spine in the prone position. Press the HT 01 point at the axilla center while the subject is in a supine position. Then press the LI 11 point in the depression medial to the extensor carpi radialis at the lateral end of the cubital crease in the lateral recumbent position.
Hold the shoulder joint using the left thumb and middle finger. Stretch the forelimb in adduction, abduction, anterior extension, and posterior extension positions for 10 seconds. Perform the procedure daily for two weeks.
After euthanizing the rat, remove the scapula in proximal 2/3s of the humerus and block ensuring exposure of the lower edge of the scapula. Next, insert an injection needle along the humeral shaft, positioning it into the humeral head. Insert two injection needles vertically into the upper and lower corners of the scapula on plastic foam wrapped with a sterile surgical sheet.
Attach a thin thread to the injection needle on the humeral shaft. Next, pull the thread at the other end with a five gram force ensuring it is parallel to the humeral shaft. Finally, measure the angle between the lower edge of the scapula and the humeral shaft.
The glenohumeral ROM of rats in the M group was significantly lower than that in the C group. Moreover, the ROM in the MT group and MO group was significantly higher than that in the M group. However, the ROM in the MO group was significantly lower than that in the MT group.
