Shandong University of Traditional Chinese Medicine

9 ARTICLES PUBLISHED IN JoVE

Behavior

Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation

Ke Ma¹, Dominique P. Lippelt¹, Bernhard Hommel¹

¹Cognitive Psychology Unit, Leiden University

Here, we describe virtual-hand and virtual-face illusion paradigms that can be used to study body-related self-perception/-representation. They have already been used in various studies to demonstrate that, under specific conditions, a virtual hand or face can be incorporated into one's body representation, suggesting that body representations are rather flexible.

Biochemistry

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

Bing Sun¹, Yue-zhang Yin², Jing Xiao¹

¹Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College, ²Shandong University of Traditional Chinese Medicine

Clinically, estrogen deficiency in menopausal women may aggravate the incidence of lipid disruption and atherosclerosis. We established an in vivo estrogen deficiency model by bilateral ovariectomy via a double dorsal-lateral incision in apoE^-/- mice. The mouse model is applicable for screening exogenous estrogen treatments of cardiovascular dysfunction after menopause.

Medicine

A New Model of Heart Failure Post-Myocardial Infarction in the Rat

Honglei Li^1,2, Jiansheng Huang³, Caihong Liu¹, Zhentao Zhang³, Kuiquan Song², Ke Ma¹, Connor W. Dennewitz³, Shijun Wang^1,4

¹College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, ²Department of Intensive Care Unit, Jinan Municipal Hospital of Traditional Chinese Medicine, ³Department of Medicine, Vanderbilt University Medical Center, ⁴Shandong Co-Innovation Center of Classic TCM Formula, Shandong Provincial Education Department

We successfully developed a reliable and reproducible model of heart failure post-myocardial infarction in the rat without ventilation or exteriorization of the heart. This simplifies the procedure and benefits the further studies about the potential mechanisms behind the heart failure.

Biology

Surgically Induced Cardiac Volume Overload by Aortic Regurgitation in Mouse

Jian Wu^*1, Shijun Wang^*1, Le Kang^*1, Chunjie Yang^*1, Jianguo Jia¹, Xingxu Wang², Ge Wei¹, Xuan Li¹, Jieyun You², Yunzeng Zou¹

¹Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, ²Department of Cardiovascular Medicine, Shanghai East Hospital, School of Medicine, Tongji University

This protocol presents a practical guide on the surgery for creation of aortic regurgitation (AR) in the mouse. Assessment of the AR mouse by echocardiography and invasive hemodynamic measurement recapitulates its clinically relevant characteristics of volume overload-induced eccentric hypertrophy, suggesting its promising application in the study of cardiac hypertrophy.

Medicine

Tuina in a Frozen Shoulder Rat Model: An Efficient and Reproducible Protocol

Yingjie Qiao¹, Yanhong Yang², Jianmin Wang³, Muzhen Li¹, Lijun Zheng¹, Huadong Li³, Shidong Zhang¹

¹College of Acupuncture and Tuina, Shandong University of Traditional Chinese Medicine, ²Department of Tuina, Jinan Massage Hospital, ³Department of Tuina, Shandong University of Traditional Chinese Medicine Affiliated Hospital

This study develops an efficient and reproducible Tuina protocol for treating frozen shoulder established in a rat model. This approach will help to study the Tuina therapy treatment method for frozen shoulders.

Immunology and Infection

Du-Moxibustion in a Mouse Model of Ankylosing Spondylitis

Wen-Yan Yu¹, Xu-Hao Li¹, Zhi-Xia Jia², Yi Hou¹, Tian-tian Dong³, Xing-Xin Wang¹, Yuan-Xiang Liu², Ji-Guo Yang¹

¹School of Acupuncture-Moxibustion and Tuina, Shandong University of Traditional Chinese Medicine, ²School of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, ³External Treatment Center, Affiliated Hospital of Shandong University of Traditional Chinese Medicine

This paper describes in detail the operation procedures and precautions to be taken during Du-moxibustion in the treatment of ankylosing spondylitis in experimental mice.

Medicine

Tuina Intervention in Rabbit Model of Knee Osteoarthritis

Shidong Zhang^*1, Xuan Zhang^*2, Gangyi Sun³, Kai Wang¹, Yingjie Qiao¹, Yanling He³, Muzhen Li¹, Huadong Li³, Lijun Zheng³

¹Shandong University of Traditional Chinese Medicine, ²Liaoning University of Traditional Chinese Medicine, ³Shandong University of Traditional Chinese Medicine Affiliated Hospital

The protocol describes a method for Tuina intervention in a rabbit model of knee osteoarthritis.

Medicine

Correction of Presbyopia by Monocular Bi-Aspheric Ablation Profile

Xiaofan Wang¹, Li Li¹, Qingbao Wang¹, Hongsheng Bi², Hua Fan², Xingrong Wang², Peng Ji²

¹Shandong University of Traditional Chinese Medicine, ²Shandong Clinical Medicine Research Center, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine

This article provides a step-by-step guide to correcting presbyopia with a monocular bi-aspheric ablation profile.

Medicine

Network Pharmacology and Validation of the Antidepressant Mechanisms of Qiangzhifang in a Chronic Restraint Stress-induced Depression Rat Model

Wanqing Ren^*1, Tongzheng Liu^*2, Jie Liu³, Jianlin Wu¹

¹College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, ²Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, ³Office of Shandong College of Traditional Chinese Medicine

This study evaluated the antidepressant efficacy of Qiangzhifang in a rat model of chronic restraint stress-induced depression and elucidated its regulatory effect on HIF-1 and JAK-STAT pathways by network pharmacology and molecular docking analysis.