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02:52 min
February 9th, 2024
DOI :
10.3791/200382-v
Transcript
首先,对先前在脑室内注射释放鸡尾酒的麻醉大鼠进行皮下镇痛。将大鼠安装在立体定位框架上。使用耳杆固定头部,确保向前和向后旋转运动不受阻碍。
将头部向下稳定 40 度角,以适当伸展颈部后部。用剃须刀剃掉颈部毛发,然后用无菌棉签交替用三轮 10% 聚维酮碘和酒精对皮肤进行消毒,每次向不同方向摩擦三到五秒钟。用指尖识别枕骨突起和寰椎之间的圆形可抑郁区域。
用记号笔标记这个点。将一毫升注射器固定在立体定位框架上。将针头定位以接触标记处的皮肤。
使用镊子,抬起皮肤,
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