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Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis (Video) | JoVE

DOI :

10.3791/200520-v

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5:25 min

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January 12th, 2024

January 12th, 2024

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196 Views
1Department of Biomedical Engineering, University of Rochester, 2Theodor Kocher Institute, University of Bern, 3Department of Biomedical Engineering, Rochester Institute of Technology

Tags

Keywords Blood brain Barrier
Monoculture
SiM Device
Immunocytochemistry
Confocal Analysis
Cell Culture
Collagen
Fibronectin
HECSR
EECM BMEC
Cell Detachment
Endothelial Medium
Methanol Fixation
PBS Washing
Blocking
Primary Antibody
Secondary Antibody
Hoechst Staining

-- Views

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