Name: Video: Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation
Uploaded: 2023-09-22T14:20:00
Duration: 2 min 41 s
Description: 522 Views. Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation

midline laparotomy surgery in donor mice for orthotopic liver transplantation

Liver Transplantation in Mouse and Analyzing Allograft Patency

Transplantation is the only effective therapy for end-stage liver disease. Undeniably, the benefit of liver transplantation is excellent, with a median survival of 11.6 years versus 3.1 years on the waiting list1. However, there are significant constraints, which limit the broad application of liver transplantation, and include most importantly, a lack of suitable, high-quality donor organs. Expanding the donor organ pool will, thus, require innovative strategies that allow for the use of allografts currently considered unsuitable today, increasing the margin of safety for transplantation. Therefore, to improve access to liver transplantation, it is imperative to conduct preclinical studies in small animals.
Particularly important to transplantation research are in vivo models of transplantation. Mouse orthotopic liver transplantation (OLT) has been around for almost 30 years2 and is vital to studying many aspects of transplantation, including the characterization of immune responses, ischemia-reperfusion injury, acute rejection, therapeutic effects of novel agents, and long-term survival3,4,5,6,7. The use of mice to study transplantation is vital as it allows for the use of transgenic mouse lines to study the impact of specific molecular pathways on the outcomes of transplantation. Established protocols of mouse liver transplantation have been well described previously8,9.
Multiple methods of anastomoses exist for the supra and infra hepatic inferior vena cava (IVC), portal vein (PV), and common bile duct (CBD). They typically rely on either hand anastomosis or a modified vascular cuff technique similar to murine lung transplantation10,11,12. An important step in the long-term study and survival of the recipient mice, as well as the development of a sustained mouse liver transplant program, is the ability to evaluate these critical anastomoses. Imaging modalities to evaluate liver allograft patency often rely on ultrasound and computer tomography (CT) in the clinical setting13,14. CT has a distinct advantage over ultrasound as it can offer views of the entire abdomen to include every anastomosis, although obtaining these views with ultrasound can be particularly difficult in small animals. Significant research and resources have been devoted to developing accurate microCT for the purpose of enhancing animal studies and the information we can gather from these models of injury and disease15,16. Here we describe a protocol for orthotopic mouse liver transplantation (Figure 1) and briefly describe a protocol for microCT to evaluate allograft patency and durability of anastomoses.

Author Spotlight: Enhancing Transplantation Research Through MicroCT Angiography in Murine Models 

Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography

Watch this Scientific Journal Video about Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation at JoVE.com

Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation   

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522 Views. Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation

Video: Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation   

Microcomputed tomography (microCT) angiography is an invaluable resource to researchers. New advances in this technology have allowed for high-quality images to be obtained of micro-vasculature and are high-fidelity tools in the field of organ transplantation. In this model of orthotopic liver transplantation (OLT) in mice, microCT affords the opportunity to evaluate allograft anastomosis in real time and has the added benefit of not having to sacrifice study animals. The choice of contrast, as well as image acquisition settings, create a high-definition image, which gives researchers invaluable information. This allows for evaluation of the technical aspects of the procedure as well as potentially evaluating different therapeutics over an extended duration of time. In this protocol, we detail an OLT model in mice in a stepwise fashion and finally describe a microCT protocol that can give high-quality images, which aid researchers in in-depth analysis of solid organ transplantation. We provide a step-by-step guide for liver transplantation in a mouse, as well as briefly discuss a protocol for evaluating the patency of the graft through microCT angiography.

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.

Microcomputed tomography (microCT) angiography is an invaluable resource to researchers. New advances in this technology have allowed for high-quality ...