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Please note that all translations are automatically generated. Click here for the English version.
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01:33 min
August 18th, 2023
DOI :
10.3791/200633-v
Transcript
首先,使用塑料巴斯德移液管轻轻地将荧光染色的脑切片转移到玻璃底共聚焦培养皿中。用平衡的ACSF填充这些盘子。用铁网将大鼠脑切片固定到位。
接下来,将玻璃底部共聚焦培养皿放在共聚焦显微镜的载物台上。将急性脑切片放置在培养皿底部,并在成像过程中灌注平衡的ACSF。使用 40X 物镜,查看大脑皮质微血管系统的壁细胞。
接下来,使用兼容的软件捕获图像堆栈。根据脑微血管系统和周细胞的网络和形态识别它们,然后在每个脑切片上定位包含脑微血管系统网络的特定区域并捕获图像。在正常生理
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