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02:19 min
June 23rd, 2023
DOI :
10.3791/201004-v
Transcript
首先,取野生型和 CENP-E 突变体 HeLa 细胞培养物,在孵育 5 小时之前加入 300 纳摩尔秋水仙碱。接下来,将细胞与0.25%胰蛋白酶-EDTA在37摄氏度下孵育3分钟,并将细胞收集到1.5毫升离心管中。接下来,在室温下以1000G离心细胞5分钟,弃去上清液并加入1.2毫升0.075摩尔氯化钾溶液。
将细胞在 37 摄氏度下孵育 20 分钟。在孵育结束时,离心细胞,弃去上清液,并加入0.2毫升固定溶液作为前缀。轻轻混合一分钟,然后如前所述收集细胞沉淀,并在室温下加入
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