Name: isolating stem cells from soft musculoskeletal tissues
Uploaded: 2010-07-05T17:00:00
Description: Watch this Scientific Journal Video about Isolating Stem Cells from Soft Musculoskeletal Tissues at JoVE.com

The authors are greatly appreciative of Ms. Haiying Pan and Mr. Ian Bellayr for their technical help on cell isolation for the current films and Mr. Kyle Holden and Mr. Jonathan Lucas for their help on slide editing. Many thanks to the following list of persons who have been involved with the development of the modified preplate techniques over the past years: Drs. Burhan Gharaibeh, BaoHong Cao, Deasy Bridget, Thomas R. Payne, Aiping Lu, Hairong Peng, Hideki Oshima, Bo Zeng, Xiaodong Mu, Kimimasa Tobita, Ron Jankowski, Makato Ikezawa. We are grateful for the technical assistance from James H. Cummins, Ryan Pruchnic, Joseph Feduska, Rebecca C. Schugar, Haiying Pan and Jessica Tebbets. The author also would like to acknowledge the financial support for this work from the Muscular Dystrophy Association (MDA), the National Institutes of Health (NIH), the Department of Defense (DOD), the Children's Hospital of Pittsburgh of UPMC, the Department of Orthopaedic Surgery, and the University of Pittsburgh.

सामान्य में, पूरा संशोधित preplate प्रक्रिया की तैयारी के 1-2 दिन, तकनीकी प्रक्रिया के प्रदर्शन के एक सप्ताह, immunocytochemistry के साथ सेल की पहचान के 2-3 दिनों के, और स्टेम सेल की आबादी विस्तार के एक अतिरिक्त 2-3 सप्ताह की आव?...

<ol>
	<li>Gharaibeh, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+of+a+slowly+adhering+cell+fraction+containing+stem+cells+from+murine+skeletal+muscle+by+the+preplate+technique.">Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique.</a> Nat Protoc. 3, 1501-1509 (2008).</li><li>Qu-Petersen, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+a+novel+population+of+muscle+stem+cells+in+mice:+potential+for+muscle+regeneration.">Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration.</a> J Cell Biol. 157, 851-864 (2002).</li><li>Li, Y. &. a. m. p. ;. a. m. p., Huard, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differentiation+of+muscle-derived+cells+into+myofibroblasts+in+injured+skeletal+muscle.">Differentiation of muscle-derived cells into myofibroblasts in injured skeletal muscle.</a> Am J Pathol. 161, 895-907 (2002).</li><li>Watt, D. J., Lambert, K., Morgan, J. E., Partridge, T. A. &. a. m. p. ;. a. m. p., Sloper, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Incorporation+of+donor+muscle+precursor+cells+into+an+area+of+muscle+regeneration+in+the+host+mouse.">Incorporation of donor muscle precursor cells into an area of muscle regeneration in the host mouse.</a> J Neurol Sci. 57, 319-331 (1982).</li><li>Lu, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+of+myogenic+progenitor+populations+from+Pax7-deficient+skeletal+muscle+based+on+adhesion+characteristics.">Isolation of myogenic progenitor populations from Pax7-deficient skeletal muscle based on adhesion characteristics.</a> Gene Ther. 15, 1116-1125 (2008).</li><li>Lee, J. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clonal+isolation+of+muscle-derived+cells+capable+of+enhancing+muscle+regeneration+and+bone+healing.">Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing.</a> J Cell Biol. 150, 1085-1100 (2000).</li><li>Cao, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+receptors+in+the+maturation-dependent+adenoviral+transduction+of+myofibers.">The role of receptors in the maturation-dependent adenoviral transduction of myofibers.</a> Gene Ther. 8, 627-637 (2001).</li><li>Cao, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Muscle+stem+cells+differentiate+into+haematopoietic+lineages+but+retain+myogenic+potential.">Muscle stem cells differentiate into haematopoietic lineages but retain myogenic potential.</a> Nat Cell Biol. 5, 640-646 (2003).</li><li>Huard, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regenerative+medicine+based+on+muscle+stem+cells.">Regenerative medicine based on muscle stem cells.</a> J Musculoskelet Neuronal Interact. 8, (2008).</li><li>Gates, C. B., Karthikeyan, T., Fu, F. &. a. m. p. ;. a. m. p., Huard, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regenerative+medicine+for+the+musculoskeletal+system+based+on+muscle-derived+stem+cells.">Regenerative medicine for the musculoskeletal system based on muscle-derived stem cells.</a> J Am Acad Orthop Surg. 16, 68-76 (2008).</li></ol>

यह स्वीकार किया गया है कि कुछ वयस्क ऊतकों एकाधिक स्टेम कोशिकाओं होते. अध्ययन के पिछले कुछ वर्षों में स्टेम कोशिकाओं के कंकाल की मांसपेशी और कण्डरा सहित नरम musculoskeletal ऊतकों में पाया गया है. यह वर्तमान प्रोट...

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isolating stem cells from soft musculoskeletal tissues

वयस्क स्टेम कोशिकाओं को लंबे समय से पुनर्योजी चिकित्सा में अपने आवेदन के संबंध में चर्चा किया गया है. वयस्क स्टेम कोशिकाओं उनके प्रभावशाली क्षमताओं के कारण घायल हो गए और रोगग्रस्त ऊतकों के उपचार के लिए बहु - वंश सेल भेदभाव और उनके आत्म नवीकरण क्षमता से गुजरना करने के लिए उत्साह का एक बड़ा सौदा उत्पन्न किया है. सबसे महत्वपूर्ण बात, इन गुणों उन्हें ऑटोलॉगस कोशिका प्रत्यारोपण चिकित्सा में उपयोग के लिए फायदेमंद बना दिया है. वर्तमान प्रोटोकॉल पाठकों को संशोधित preplate तकनीक जहां musculoskeletal प्रणाली है, जैसे पट्टा और मांसपेशियों के कोमल ऊतकों हैं 1 से मिलवा देंगे<sup&gt; सेंट</sup&gt; Enzymatically dissociated और तो माध्यम के साथ कोलेजन लेपित बोतल में रखा. सतह पर तैरनेवाला, जो मध्यम और शेष अस्थायी कोशिकाओं से बना है serially नई बोतल के लिए दैनिक स्थानांतरित किया है. स्टेम सेल बोतल जो आमतौर पर 5-7 दिन लगते है (धारावाहिक स्थानान्तरण या preplates) का पालन करने के लिए धीमी कर रहे हैं. इस तकनीक का उपयोग करके, वयस्क स्टेम सेल इन ऊतकों में उपस्थित, काफी गैर इनवेसिव प्रक्रियाओं के माध्यम से आसानी से काटा जा सकता है है.

Watch this Scientific Journal Video about Isolating Stem Cells from Soft Musculoskeletal Tissues at JoVE.com

Isolating Stem Cells from Soft Musculoskeletal Tissues

Musculoskeletal मुलायम ऊतकों से अलग वयस्क स्टेम सेल का पालन फ्लास्क करने की गति के आधार पर कोशिकाओं.

शीतल Musculoskeletal ऊतकों से स्टेम कोशिकाओं को अलग

Adult stem cells have long been discussed in regards to their application in regenerative medicine. Adult stem cells have generated a great deal of ...

Research

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Biology

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Murine भ्रूण स्टेम कोशिकाओं से hematopoietic स्टेम सेल की व्युत्पत्ति

A stem cell is defined as a cell with the capacity to both self-renew and generate multiple differentiated progeny.  Embryonic stem cells (ESC) are ...

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

अलगाव और अपरा से hematopoietic स्टेम सेल का विश्लेषण

Hematopoietic stem cells (HSCs) have the ability to self-renew and generate all cell types of the blood lineages throughout the lifetime of an individual. ...

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

मानव भ्रूण स्टेम कोशिकाओं से chromatin immunoprecipitation

The functional and structural complexity of the myriad of cells in metazoan organisms arises from a small number of stem cells. Stem cells are ...

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

Blastocysts से माउस ट्रोफोब्लास्ट स्टेम सेल की व्युत्पत्ति

Specification of the trophectoderm is one of the earliest differentiation events of mammalian development. The trophoblast lineage derived from the ...

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

मानव अग्नाशय के कैंसर xenografts से स्टेम सेल के अलगाव

Cancer stem cells (CSCs) have been identified in a growing number of malignancies and are functionally defined by their ability to undergo self-renewal ...

Isolation of Retinal Stem Cells from the Mouse Eye

माउस आँखों से रेटिना स्टेम सेल के अलगाव

The adult mouse retinal stem cell (RSC) is a rare quiescent cell found within the ciliary epithelium (CE) of the mammalian eye1,2,3. The CE is made up of ...

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

मानव प्रेरित pluripotent स्टेम सेल का चयन और अलग कालोनियों वयस्क Fibroblasts से reprogrammed किया

Herein we present a protocol of reprogramming human adult fibroblasts into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

प्रेरित pluripotent स्टेम सेल से व्युत्पन्न चूहों की पीढ़ी

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

Isolating Primary Melanocyte-like Cells from the Mouse Heart

माउस दिल से प्राथमिक Melanocyte कोशिकाओं की तरह अलग

We identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial ...

Induction and Analysis of Epithelial to Mesenchymal Transition

Mesenchymal संक्रमण के लिए प्रेरण और उपकला का विश्लेषण

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...