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02:15 min
January 19th, 2024
DOI :
10.3791/201369-v
* These authors contributed equally
Transcript
首先,启动流式细胞仪软件。打开一个新实验。添加所需数量的样本并命名它们。
然后设置 YL2-H 或 RL1-H 通道直方图、前向散射面积与侧向散射面积点图、前向散射面积与前向散射高度点图以及 BL1-H 与 VL2-H 通道点图。将未染色的野生型布氏锥虫对照细胞放在样品进样口上,并调整载物台位置。要减小文件大小,请取消选中不打算录制的通道。
从低流速开始,以便进行调整。然后单击“运行”开始数据采集。微调 YL2 或 RL1 电压,使 10 以内的主峰与第三至 10
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