stimuli procedure for measuring visual motion acuity on random dot kinematograms

通过随机点运动图中的形状识别评估视觉

Most of the available visual tests are directed to examine the features processed by central vision, relying on the input deriving from the central retina1. The central retina has the densest cone-photoreceptor population for maximal visual acuity and lacks rod photoreceptors, which dominate the peripheral retina2. The presence of densely packed photoreceptors is also reflected in an increased density of ganglion cells, which means a greater number of axons are directed to the optic nerve and, eventually, to the visual cortex. Outside the fovea towards the periphery, rods outnumber the cone photoreceptor3. With the rods&#39; wider bodies and the sparser mosaic of photoreceptors, the peripheral retina is primarily responsive to night vision and motion awareness4.
Classically, it was believed that visual processing, depending on stimulation of the central part of the visual field, is devoted to the fine analysis of stationary objects, and its peripheral part is specialized in detecting motion and bringing objects to the central, foveal vision, where it is further analyzed5,6. However, now we have emerging evidence showing that on the cortical level, the fine analysis of the stationary pathway is not fully separated from the motion-sensitive one6,7,8. Testing form and motion perception simultaneously is classically performed using moving gratings9 and glass patterns10 and also concentric rings motion11. Our goal is to introduce a test that is close to the normal life of visually disabled people, which can lower their frustrations and give hope by explicitly showing them that some features of their visual processing might still be preserved and even strengthened. The proposed motion-acuity test based on random dot kinematograms (RDKs) combines motion and shape perception analysis and simultaneously tests the functioning of the motion and shape perception. Within the motion-acuity test, there are many possibilities of psychophysical features to test, such as different velocities, directions, and contrasts of the RDKs. By changing the parameters, we can manipulate the strength of stimulation, either specific for the central processing or peripheral. For example, detecting fast-moving objects is a well-described feature specific to peripheral visual processing12, whereas the processing of the darks on the bright background is preferentially processed by central vision13. This test was initially performed on patients with retinal degeneration of photoreceptors, either specifically located within the central or peripheral retina14. Retinitis pigmentosa (RP) is manifested with peripheral damage and prevails in ~1/5000 patients worldwide15. Stargardt disease (STGD), with a prevalence of ~1/10000, is the most common cause of juvenile macular degeneration (MD)16. Damage to the photoreceptors at the central retina, as in macular degeneration or as in retinitis pigmentosa at the peripheral retina, results in corresponding visual field losses. Those visual field losses are reflected in the impairments of the features specific to the given visual system regions17. Importantly, the regions of the visual system that receive inputs from unaffected parts of the retina are also affected. It was previously shown in animal models of macular degeneration18 that after binocular central retinal damage, not only is acuity aggravated, but motion perception, a feature characteristic of peripheral processing, is reinforced. The motion-acuity testing described here provides an important insight for planning visual rehabilitation procedures. A complete view of the interplay between central and peripheral parts of the visual field has a crucial role in understanding how lost functions might be taken over by the spare parts of the visual system and how this process can be supported by visual training rehabilitation procedures. Inline, the knowledge of how regional retinal degeneration affects visual processing, especially beyond its damaged parts, still remains incomplete. Optical tests are based on the measurements of the stationary shape features. For instance, the visual acuity measurements rely on stationary stimuli, either letters (Snellen charts), grating charts, or vernier acuity charts.
For the purpose of widening the insight into the dynamics between central and peripheral vision in healthy eyes and eyes that have impaired central/peripheral visual functions, a motion-based acuity test measuring the shape and motion perception simultaneously was introduced. The motion-acuity test is based on the detection of centrally located shapes in negative or positive contrast (dark or light dots), an ellipse and circle with matching surfaces, built from random dot kinematograms (RDK) and separated from the same RDK background by velocity, coherence, or direction. Acuity is measured as the minimal difference perceived between circle and ellipse dimensions, and the results are given in visual degrees at which the subject stops to perceive the difference. Additionally, to check if the luminance contrast influences the measured motion acuity, stimuli can be presented in negative (black dots on white background) or in positive contrast (white dots on black background). All the available information about positive contrast (ON type) and negative contrast (OFF type) processing in the visual system comes from the stationary stimulation of the central visual field19,20. But how peripheral processing of motion signals depends on contrast remains fairly unknown14,21. It was only established that sensitivity to high velocities is specific for peripheral processing, whereas central motion processing engages slow velocities at higher spatial frequencies presented in positive contrast (ON type)12. The positive and negative contrast versions of the motion-acuity stimuli, as well as the ability to modify the velocity of dots, as well as the coherence or direction, is crucial for a more detailed description of the full visual field. Additionally, a mechanical narrowing of the visual field to central 10 degrees is proposed using goggles with lenses replaced with opaque ones with centrally located holes. This easily replicable narrowing system, suitable for fMRI and TMS protocols, allows further investigations of the functions of the peripheral visual input and how visual peripheries influence centrally measured acuity. A similar system was initially validated in previous studies14, in which it was found that motion acuity tests in negative contrast and in fast motion, strongly activating visual peripheries, are the most difficult for all participants. For patients with Stargardt disease, they were unmanageable. Importantly, attenuation of visual periphery stimulation, by diminishing the velocity of RDKs, improves acuity thresholds in all tested subjects. In conclusion, we propose the task with motion-acuity measurement based on simple shape discrimination. Therefore, the results are straightforward and easy to understand also for patients and their caregivers. The motion-acuity test presented here is also addressed for users outside academia. The task is easy to explain to a broad range of ages and patient groups.

作者聚焦：通过基于运动的周边视觉测试评估中央视力丧失患者的视力

运动敏锐度测试，用于使用运动定义形状的视野敏锐度测量

We are searching for the potentially-enhanced visual functions in patients with injured visual system. Our research suggests that peripheral vision with its sensitivity to motion may be able to partially compensate for the loss of the central vision. Optical tests are based on the measurements of the stationary shape features.We are proposing to measure acuity, the sharp vision with shapes of an equal surface built from moving dots. For the first time, it is possible to measure acuity based on motion sensitivity. The proposed protocol offers an accessible tool to assess visual acuity by recruiting central and peripheral visual fields.Also, the possibility to use positive or negative contrast to modify the velocity of dots and the coherence or direction is crucial for a more detailed description of the full visual field. An assessment of the interplay between central and peripheral parts of the visual field has a crucial role in understanding how lost functions might be taken over by the spare parts of the visual system, and how this process can be supported by visual training rehabilitation procedures.

在随机点运动图上测量视觉运动敏锐度的刺激程序

首先，安排运动敏锐度测试的设置并执行测试以确定其难度级别。然后，一旦主题准备好，打开配置文本文件以继续进行相干性测试。注释掉读取 filename-Shape_Brt 的行。txt。之后，取消注释其下方的行，其中包括Shape_dotsB_C. txt任务。执行 Viscacha2.jar 程序。只需简化主题的任务说明即可。参与者准备就绪后，按 S 键开始实验。然后，重新打开配置文本文件。注释掉 filename-shape_dotsB_C 行。txt 并取消注释包含shape_dotsW_C. txt任务的行。对于方向任务，在打开配置文本文件后，对先前选定的文件名进行注释，并取消对包含 filename-shape_dotsB_D 的行的注释。txt 任务。执行 Viscacha2.jar 程序。向参与者解释任务，然后按 S 键开始实验。然后重新打开配置文本文件。注释掉 Filename-shape_dotsB_D 行。txt 并取消注释下面的行，其中包括shape_dotsW_D. txt任务。对于速度任务，打开配置文本文件并在先前选择的文件名上添加注释。取消对包含 filename-shape_dotsB_V1020 的行的注释。txt 任务。执行 Viscacha2.jar 程序。向参与者解释任务，然后按 S 键开始实验。然后注释掉shape_dotsB_V1020行。txt 并取消注释其下方的行，其中包括shape_dotsW_V1020。txt 任务。

Research

JoVE Journal

Neuroscience

Stimuli Procedure for Measuring Visual Motion Acuity on Random Dot Kinematograms

Motion-Acuity Test for Visual Field Acuity Measurement with Motion-Defined Shapes

The standard visual acuity measurements rely on stationary stimuli, either letters (Snellen charts), vertical lines (vernier acuity) or grating charts, ...

科研

神经科学

Determining the Initial Difficulty of the Motion-Based Acuity Test

确定基于运动的视力测试的初始难度 