Medical University of Warsaw

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture

Adrianna Maria Piasek^*1, Iryna Levkovych^*1, Paulina Musolf¹, Hanna Chmielewska¹, Aneta Ścieżyńska², Anna Sobiepanek¹

¹Chair of Drug and Cosmetics Biotechnology, Faculty of Chemistry, Warsaw University of Technology, ²Department of Histology and Embryology, Medical University of Warsaw

Here, we present inexpensive and simple procedures to introduce various 3D skin models for routine research in a cell culture laboratory. Researchers can create models tailored to their needs without relying on commercially available models.

Neuroscience

Motion-Acuity Test for Visual Field Acuity Measurement with Motion-Defined Shapes

Marco Ninghetto¹, Michał Wieteska^2,3, Anna Kozak¹, Kamil Szulborski⁴, Tomasz Gałecki⁴, Jacek Szaflik⁴, Kalina Burnat¹

¹Nencki Institute of Experimental Biology, Polish Academy of Sciences, ²Mossakowski Medical Research Institute, Polish Academy of Sciences, ³Institute of Radio Electronics and Multimedia Technology, Warsaw University of Technology, ⁴Department of Ophthalmology, Medical University of Warsaw

A novel motion-based acuity test that allows the assessment of central and peripheral visual processing in low-vision and healthy individuals, along with goggles limiting peripheral vision compatible with MRI protocols, are described here. This method offers a comprehensive vision assessment for functional impairments and dysfunctions of the visual system.

Medicine

Preparation and Analysis of Histological Slides of Rat and Mouse Eyeballs to Evaluate the Retina

Ewa Sikorska^*1, Dominika Wołosz^*2, Kaja Kasarełło¹, Łukasz Koperski², Barbara Górnicka², Agnieszka Cudnoch-Jędrzejewska¹

¹Chair and Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, ²Department of Pathology, Medical University of Warsaw

This manuscript presents a protocol for the histological preparation of slides from rodent eyeballs. With the proposed method, the inner retina can be easily visualized and assessed under a light microscope. The procedure is presented for the eyeballs of mice and rats.