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01:56 min
February 16th, 2024
DOI :
10.3791/201541-v
Transcript
首先,将每种细菌菌株的计算量混合,使每毫升总共达到约 500 万个菌落形成单位。将混合物以 7, 000 G 在 4 摄氏度下离心 7 分钟。接下来,小心地用移液液出上清液。
将沉淀重新悬浮在缓冲能力和葡萄糖含量低的 Hank 平衡盐溶液中。现在,从豆荚中分离出含有分化的人阴道上皮细胞的器官芯片。用移液器吸头塞住芯片基底通道的出口。
将 200 μL 无抗生素分化培养基加入基底通道入口,而不向下推移液器柱塞。从移液器中松开移液器吸头,让培养基自由流过通道。接下来,将
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