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Closed Circular Extrachromosomal Ribosomal DNA (rDNA) Containing Element (CERE) Isolation from Transfected Naegleria Gruberi Trophozoites and PCR Analysis

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02:27 min

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June 21st, 2024

DOI :

10.3791/202099-v

June 21st, 2024

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Brian T. Nguyen¹, Nora M. Chapman², John C. Mullican³, Kristen M. Drescher¹

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine, ²Department of Pathology and Microbiology, University of Nebraska Medical Center, ³Department of Biology, Washburn University

Transcript

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Closed Circular Extrachromosomal Ribosomal DNA

Naegleria Gruberi

Sodium Chloride

Ammonium Acetate

Absolute Ethanol

Gel Electrophoresis

Ultraviolet Light System

Plasmid Retention

From the series

Using a CERE-Based Vector for Transfection of Naegleria gruberi Trophozoites

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