Hardware Assembly to Perform Transcranial Photobiomodulation for Brain Drainage in Aged Mice

To begin the hardware assembly, solder the light emitting diode or LED to the printed circuit board or PCB, and place the LED and magnets on the 3D frame. Then connect the resistor between an LED anode and the five volt port on the Arduino. Next, connect an LED cathode to the metal oxide semiconductor field effect transistor or MOSFET drain, and secure the MOSFET source to the ground.

After connecting the pull down resistor between the MOSFET gate and the ground, proceed to pin the MOSFET gate to pin three on the Arduino. Then connect a liquid crystal display or LCD keypad shield to the Arduino. And insert the Arduino board with LCD keypad shield MOSFET and LED connector into the case.

Finally, download the Arduino sketch and open it via the Arduino integrated development environment. Select the correct communication port and flash the firmware.