JoVE Logo

Sign In

Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

1.1K Views

06:03 min

September 20th, 2024

DOI :

10.3791/66593-v

September 20th, 2024


Explore More Videos

Whole Blood Assay

Chapters in this video

0:00

Introduction

1:41

Stimulation and Incubation of Whole Blood Samples for Antigen‐Specific Analysis of Host Immunity to Fungal and Viral Pathogens

3:13

Preparation of Whole Blood Samples for Flow Cytometry and Cytokine Analysis to Determine Antigen‐Specific Host Immunity to Fungal and Viral Pathogens

Related Videos

article

06:13

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

34.7K Views

article

11:55

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

14.5K Views

article

08:04

Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

15.6K Views

article

12:29

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

10.1K Views

article

11:00

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

44.8K Views

article

08:10

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

8.2K Views

article

08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

10.5K Views

article

In vivo Function of Differential Subsets of Cutaneous Dendritic Cells to Induce Th17 Immunity in Intradermal Candida albicans Infection

1.6K Views

article

07:21

Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

1.2K Views

article

08:48

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

2.8K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved