To begin, take a syringe to inject the prepared 1%Carrageenan solution. Shake the Carrageenan solution and draw it into the syringe while ensuring that there is no bubble in the syringe. Then, disinfect the injection site with 75%alcohol.
Insert the needle diagonally upwards into the hind paw foot pad while maintaining the stability of the syringe to inject 30 microliters of 1%Carrageenan solution. After injection, withdraw the syringe slowly and press on the injection site for two seconds to prevent leakage. Clean and disinfect the surface of the hot plate.
Start the hot plate apparatus and click the Temperature Mode button. Then, click Constant to set the temperature to approximately 55 degrees Celsius. Click Start Experiment button and click Reach.
Now, take a mouse from the accommodation container. Gently grasp its trunk and place it in the center of the hot plate. Then, click Start.
Observe and record the time to lift the left hind paw or hind paw in response to the heat stimulation. Press the pedal of the hot plate to see the time on the hot plate screen. Then set a 60 second limit for the hot plate test.
Record a pain threshold of 60 seconds if the limit is reached. Take one capsule, containing 200 milligrams of Celecoxib. Get the content of the capsule and grind it into a powder.
To prepare the store suspension of Celecoxib, add 0.5%sodium carboxy methylcellulose to make a final volume of 13.3 milliliters. Then take one milliliter of the store suspension of Celecoxib and add 9.0 milliliters of 0.5%sodium carboxy methylcellulose to obtain Celecoxib suspension. Administer 0.2 milliliters per 10 grams of the Celecoxib suspension, intragastrically to the mice.
Randomly divide ICR female mice into three groups, control, model and Celecoxib groups. Inject 30 microliters of 1%Carrageenan solution subcutaneously into the planter surface of the hind paw. After the injection, intragastrically administer Celecoxib at 30 milligrams per kilogram for the mice in the Celecoxib group.
Conduct the hot plate test at three hours after Carrageenan injection. The pain threshold of the single hind paw injection model did not show a significant reduction, compared with the control group. The pain threshold of the both hind paw injection model decreased significantly compared with the control group.
In the single hind paw injection model, the pain threshold of the Celecoxib group did not significantly increase, compared to the model group. However, in the both hind paw injection model, the pain threshold of the Celecoxib group showed a significant increase.
