Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse

After sacrificing an adult C57BL/6J mouse, remove the hair around the mouth with a shaver, and using scissors, cut from the cheeks toward the jaw on both sides. Next, use forceps to open the mouth wide and absorb any blood using a cotton swab. Then, disinfect the palate by wiping the inside of the mouth with a cotton swab containing 10% povidone-iodine.

To harvest the mouse palate, first, using a surgical scalpel blade, make a full-thickness marginal incision along the palate side of the maxillary teeth. Then, using a raspatorium, carefully dissect the entire palate. Quickly transfer the palate tissue to a 15-millimeter tube containing 4 milliliters of complete medium supplemented with antibiotics and antimycotics.