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Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

DOI :

10.3791/2079-v

15:55 min

August 4th, 2010

August 4th, 2010

21,895 Views

1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Department of Medicine and Genetics, Harvard Medical School

We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.

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Intravenous Microinjections

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