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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

DOI :

10.3791/61575-v

13:25 min

May 15th, 2021

May 15th, 2021

4,636 Views

1Department of Immunology, University of São Paulo, 2Department of Medicine, Nephrology Division, Federal University of São Paulo

This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

Tags

Acute Kidney Injury

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