Generating Urinary Tract Infection Model: A Technique to Administer Uropathogenic Bacteria in Mouse to Develop Urinary Tract Infection Model

Mount a sterile 24-gauge angiocatheter tip on a 100-microliter syringe. Fill the syringe with the prepared bacterial solution. Place one animal on a working surface in the supine position, and maintain a stable isoflurane anesthesia, using a nose cone during the instillation. Apply the eye ointment. Expel the residual urine by applying gentle compression, and making circular movements on the suprapubic region. Then, clean the lower abdomen with 70% ethanol.

Lubricate the catheter tip with normal saline. Put the index finger of the non-dominant hand on the abdomen and push it gently upwards. Start the catheterization of the urethra vertically in a 90-degree angle. Once resistance is encountered, tilt it horizontally before inserting it further. Perform a slow instillation of 50 microliters of the bacterial inoculum. After the instillation, keep the syringe and catheter in place for a few more seconds, and then slowly retract to prevent leakage.