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Double Fluorescence in situ Hybridization in Fresh Brain Sections

DOI :

10.3791/2102-v

August 14th, 2010

August 14th, 2010

15,746 Views

1Department of Brain and Cognitive Sciences, University of Rochester, 2Center for Visual Science, University of Rochester

This protocol involves a non-radioactive in-situ hybridization procedure that enables the simultaneous identification of two transcript species, at a single cell resolution, in thin sections of the vertebrate brain.

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Double Fluorescence In Situ Hybridization

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