Name: visualization of the embryonic nervous system in whole-mount drosophila embryos
Uploaded: 2010-12-11T12:00:00
Description: Watch this Scientific Journal Video about Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos at JoVE.com

SG是从美国纽约州立大学布法罗分校和约翰R. Oishei基金会的资金支持。

<html> 1。试剂的制备<ol><li>准备PEMFA缓冲区，使用100mm的管道，2mm的EGTA，1毫米硫酸镁 4 。缓冲区的pH值调整至7.0。 PEMFA缓冲过滤消毒和储存在4 ° C </li><li> PBT的加入为1ml准备TRITON X - 100至100ml 1X PBS（磷酸盐缓冲液）（pH值7.0）。保存在4 ° C。 </li><li>准备1X PBS（pH值7.0）0.01％NaAcide的5％BSA（牛血清白蛋白）。 </li><li>准备的盐溶液，用0.7％NaCl和去离子水0.03％的Triton X - 100。 </li><li>准备结合PEMFA?...

<ol>
	<li>Bodmer, R., Barbel, S., Sheperd, S., Jack, J. W., Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transformation+of+sensory+organs+by+mutations+of+the+cut+locus+of+D.+melanogaster.">Transformation of sensory organs by mutations of the cut locus of D. melanogaster.</a> Cell. 51, 293-307 (1987).</li><li>Bodmer, R., Carretto, R., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurogenesis+of+the+peripheral+nervous+system+in+Drosophila+embryos:+DNA+replication+patterns+and+cell+lineages.">Neurogenesis of the peripheral nervous system in Drosophila embryos: DNA replication patterns and cell lineages.</a> Neuron. 3, 21-32 (1989).</li><li>Campos-Ortega, J. A., Hartenstein, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Embryonic Development of Drosophila melanogaster. , (1985).</li><li>Carroll, S. B., Scott, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Localization+of+the+fushi+tarazu+protein+during+Drosophila+embryogenesis.">Localization of the fushi tarazu protein during Drosophila embryogenesis.</a> Cell. 43, 47-57 (1985).</li><li>Dambly-Chaudi&#232;re, C., Ghysen, A., Jan, Y. N., Jan, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Muscle+connections+between+imaginal+discs+in+Drosophila.">Muscle connections between imaginal discs in Drosophila.</a> Developmental Biology. 113, 288-294 (1986).</li></ol>

果蝇胚胎是一个功能强大的系统，为在神经元发育的调查细胞和分子的变化。在这个协议中，我们已经演示了如何准备整装胚胎进行免疫组化。卡罗尔和斯科特，1985年的协议，我们已经适应了这个协议。该协议还可以适应测试其他神经元抗体，但抗体的优化应该做。此外，胚胎PNS和中枢神经系统的发育缺陷，可以很容易地通过比较不同的突变品系的胚胎可视化。我们已经成功地使用这一?...

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<td>37% formaldehyde</td>
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<td>Developmental Studies Hybridoma Bank</td>
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<td>Jackson ImmunoResearch Laboratories</td>
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visualization of the embryonic nervous system in whole-mount drosophila embryos

果蝇的胚胎是有吸引力的模型系统，为研究神经元发育的细胞和分子基础。在这里，我们描述了果蝇胚胎神经系统使用神经元蛋白的抗体的可视化的过程。由于整个胚胎周围神经和中枢神经系统，在单个细胞水平（Dambly - Chaudière等，1986;。博德默等，1987;。博德默等 ，1989），这些系统的像差以及特点可以很容易地确定使用不同的神经元蛋白的抗体。胚胎发育是在特定的时间收集，以确保在适当的可视化的发展阶段的胚胎。集合后，胚胎的外层，蛋壳膜和周围胚胎的卵黄信封，被删除前固定。胚胎，然后与神经细胞抗体培养和可视化使用荧光标记的抗体。在阶段的胚胎12-17可视化访问胚胎的神经系统。阶段12的中枢神经系统生殖乐队开始阶段15的缩短和明确的格局已经实现的合缝。通过阶段17中枢神经系统的合同和PNS的全面发展（坎波斯，奥尔特加等人1985年）。因此，在PNS和CNS格局的变化可以很容易地观察到在这些发育阶段。

Watch this Scientific Journal Video about Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos at JoVE.com

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

我们描述了程序准备上演<em&gt;果蝇</em&gt;胚胎神经系统的胚胎发育过程中的可视化的胚胎。

在全安装的胚胎神经系统的可视化果蝇胚胎

The Drosophila embryo is an attractive model system for investigating the cellular and molecular basis of neuronal development. Here we describe the ...

Research

JoVE Journal

Neuroscience

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos

Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection

Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection

成人整装准备果蝇巨纤维染料注射腹侧神经索

To analyze the axonal and dendritic morphology of neurons, it is essential to obtain accurate labeling of neuronal structures. Preparing well labeled ...

科研

神经科学

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

可视化本体感受器<em&gt;果蝇</em&gt;幼虫和蛹

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

标记的单细胞在中枢神经系统<em&gt;果蝇</em

In this  article we describe how to individually label neurons in the embryonic CNS of Drosophila melanogaster by juxtacellular injection of the ...

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

调查中枢神经系统修复中受伤范式<em&gt;果蝇</em

An experimental method has been developed to investigate the cellular responses to central nervous system (CNS) injury using the fruit-fly Drosophila. ...

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

啮齿动物的中枢神经系统药物直接脑室交货

Due to an inability to cross the blood brain barrier, certain drugs need to be directly delivered into the central nervous system (CNS). Our lab focuses ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

嗅觉受体神经元在全山免疫标记<em&gt;果蝇</em&gt;天线

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

在啮齿动物中枢神经系统的体内光遗传学刺激

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

纤毛对小鼠的主要嗅觉系统整装标签

The mouse olfactory system comprises 6-10 million olfactory sensory neurons in the epithelium lining the nasal cavity. Olfactory neurons extend a single ...

In Situ Ca2+ Imaging of the Enteric Nervous System

In Situ Ca2+ Imaging of the Enteric Nervous System

在肠神经系统的原位 钙成像

Reflex behaviors of the intestine are controlled by the enteric nervous system (ENS). The ENS is an integrative network of neurons and glia in two ...

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

一个简单的一步解剖协议成人全安装准备<em&gt;果蝇</em&gt;脑

There is an increasing interest in using Drosophila to model human brain degenerative diseases, map neuronal circuitries in adult brains, and study the ...