JoVE Logo
Faculty Resource Center

Sign In

Measurement of Natural Killer Cell Migration in Response to Chemotactic Stimuli


To measure NK cell migration in response to chemotactic stimuli, collect human NK cells from a 70% to 80% confluent culture, and resuspend the cells at a 2.5 x 106 cells per milliliter of serum-free NK cell culture medium concentration. Next, add 600 microliters of serum-free medium containing the NK cell chemoattractant of interest per well.

And place one 6.5-millimeter diameter culture insert with 5-micrometer pores into each well of medium. Then, add 100 microliters of NK cells to the upper compartment of each insert, and place the plate in the cell culture incubator for four hours.

At the end of the incubation, transfer the entire volume of non-adherent migrated cells from the bottom of each well into individual 5-milliliter FACS tubes, and add 15 microliters of a predetermined number of flow cytometry counting beads to each tube. Then, using a flow cytometer, evaluate each cell sample according to standard flow cytometric analysis protocols, and calculate the absolute number of migrated NK cells using the formula.

Usage Statistics

-- Views

Related Videos


Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay


Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion


A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion

JoVE Logo


Terms of Use





Copyright © 2024 MyJoVE Corporation. All rights reserved