After anesthetizing the mouse, dilate the pupil with one drop of 2.5% phenylephrine, avoiding excess droplets entering the nose or mouth. After two to three minutes, dab off the excess liquid, and cover the eye with 0.3% hypromellose gel. Next, wrap the mouse in a layer of surgical gauze to maintain body warmth, then place the mouse on the animal cassette, and position the head with the bite bar.
To acquire the optical coherence tomography, or OCT images, turn on the OCT imaging system, and open the imaging software. For posterior chamber imaging, bring the OCT close to the eye's surface, taking care not to bring the surface of the lens in contact with the eye.
Once the eye is correctly positioned, stop the Fast Scan, select the Volume Scan protocol, and activate the scan with the Aim option. For posterior segment images, adjust until the optic nerve is centered in the horizontal B-Scan alignment image and the retina is aligned with the vertical alignment axis. For anterior segment images, adjust the position to center the apex of the cornea in both the horizontal B-Scan alignment image and vertical B-Scan alignment image. Finally, click on Snapshot to capture the volume scan image. Then click on Save.
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