Take a size exclusion chromatography column packed with an inert matrix of polysaccharide resin particles. The porous particles have a defined pore size.
Flush the column with an equilibration buffer for optimal purification.
Add a concentrated culture filtrate processed from a culture of Mycobacterium tuberculosis — a pathogenic bacteria. The filtrate contains extracellular vesicles, or EVs, and other soluble proteins secreted by the bacteria.
EVs are heterogeneously sized, small, spherical structures. They contain macromolecules and virulence factors enclosed in a lipid bilayer.
The sample enters the matrix under gravity. Add the buffer to allow sample movement through the column.
The EVs cannot enter the resin due to the small pore size and instead move through the interparticle space, traveling a shorter path and eluting first.
Proteins, having a smaller size, enter the pores, travel a longer path through the resin, and elute at the end.
Collect the fractions containing EVs for downstream analysis.
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
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