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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

DOI :

10.3791/62429-v

March 31st, 2021

March 31st, 2021

3,727 Views

1Department of Chemical and Biomolecular Engineering, Lehigh University

Bacterial vesicles play important roles in pathogenesis and have promising biotechnological applications. The heterogeneity of vesicles complicates analysis and use; therefore, a simple, reproducible method to separate varying sizes of vesicles is necessary. Here, we demonstrate the use of size exclusion chromatography to separate heterogeneous vesicles produced by Aggregatibacter actinomycetemcomitans.

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