Results: Analysis of Lipid and Toxin Contents of the Collected Fractions
6:51
Conclusion
Transcript
Size exclusion chromatography is a useful approach for separating the subpopulations of heterogeneously-sized bacterial vesicles and for removing nucleic acids and proteins from bacterial supernatants. Size exclusion chromatography is less expensi
Sign in or start your free trial to access this content
Bacterial vesicles play important roles in pathogenesis and have promising biotechnological applications. The heterogeneity of vesicles complicates analysis and use; therefore, a simple, reproducible method to separate varying sizes of vesicles is necessary. Here, we demonstrate the use of size exclusion chromatography to separate heterogeneous vesicles produced by Aggregatibacter actinomycetemcomitans.