Sign In

A subscription to JoVE is required to view this content.

Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus

-- views • 1:23 min

Transcript

Take an awake rat with two guide cannulae implanted bilaterally into the hippocampus.

The cannulae are connected to subcutaneously implanted osmotic pumps containing amyloid-beta or Aβ-specific and control antibodies, respectively.

A high antibody concentration within the pump induces water diffusion from the surrounding tissue through its outer semipermeable membrane, expanding the osmotic layer.

This expansion compresses the antibody reservoir, facilitating controlled antibody release.

Attach two internal cannulae to sterile-water-filled catheters connected to an infusion pump.

Fill the cannulae with water.

Next, aspirate a small volume of air, creating a bubble.

Now, fill the cannulae with neurotoxic Aβ oligomers, soluble aggregates of Aβ peptides.

Insert the internal cannulae into the guide cannulae. Start the pump for a controlled delivery of the oligomers, using the air bubble to prevent backflow and track the infusion rate.

Administer repeated oligomer injections for the required duration.

The model is ready for studying Aβ oligomer-antibody interactions.

article

Now Playing

01:23

Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus

66 Views

article

04:07

Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus

Related Videos

28 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved