Direct Reprogramming of Hematopoietic Progenitor Cells into Neural Stem Cells

Begin with a cell suspension of genetically altered hematopoietic progenitor cells.

These cells express transcription factors required for their reprogramming into neural stem cells. This process is called direct reprogramming.

Transfer the cells to an extracellular matrix-coated multiwell plate with a neural progenitor medium, and incubate.

The extracellular matrix provides a three-dimensional scaffold for cell attachment and growth.

During incubation, transcription factors expressed by the hematopoietic progenitor cell initiate a sequence of molecular events that allow its transformation into a neural stem cell.

The neural progenitor medium contains factors that favor the survival of induced neural stem cells.

After incubation, replace this medium with a fresh neural progenitor medium to promote cell multiplication.

Detach the cells using a cell scraper.

Collect this suspension and transfer the cells to a well containing neural stem cell medium.

Incubate for maintenance of induced neural stem cells.